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The samples were drawn from an intravenous catheter located in the arm opposite to the docetaxel infusion, collected in 9 ml tubes containing EDTA as anticoagulant (Vacuette, Greiner), and placed on ice until additional processing within 60 minutes.
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Prepared isolated lymphocytes were held on ice until all samples from a patient were ready for additional processing or experimentation.
Telephone and travel agent bookings carry an additional processing fee of $8.60 a flight.
That additional processing fee can vary greatly from company to company, said Bob Carr, chief executive of Heartland Payment Systems, a payment processor.
The tickets were nonrefundable, nonchangeable and nontransferable, and there was an additional processing and shipping fee of $11.95.
No additional processing was applied.
DNA was measured as previously mentioned and stored at -20°C until further processing (Additional file 3: Table S2).
They may replace dying cells, or could be added to existing circuits to provide additional information processing capabilities.
Figure 2 Mechanical and additional pre-processing.
No additional post-processing manipulations were done to enhance images.
DNA samples were stored at −80 °C until further processing.
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