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The area under the curve (AUC) of each organ was calculated by the trapezoidal method until acquisition ended.
1.5 ml of propidium iodide staining solution was added, and the tube was incubated for 1 h at 4 °C in dark place until acquisition.
Rather, they are getting smaller amounts to see them through until acquisition or a later mega-round where the big VCs throw their weight around.
After washing, cells were fixed with PFA 1% and stored at +4°C until acquisition.
Cells were fixed in 1.0% methanol-free formaldehyde (Polysciences, Inc). in PBS containing 0.1% bovine serum albumin and refrigerated at 4.0°C until acquisition.
The samples were maintained on ice until acquisition.
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