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The working electrode was poised at a reducing potential of −0.36 V versus SHE and current was monitored until a steady baseline was reached (∼1 hour).
The column was washed with wash buffer after being loaded until a steady baseline was reached (absorbance of 280 nm).
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After sample loading, the column was washed with 10 CVs of Wash buffer (50 mM sodium phosphate, 300 mM NaCl, 5 mM imidazole, pH 7.4) until the absorbance reached a steady baseline.
The two players then settled into a steady baseline rhythm, each answering a grunt and a ground stroke with a grunt and a ground stroke.
All rats underwent a 3-day training program, by which time a steady baseline level of performance was attained.
Initially, the hindlimb was perfused with buffer alone (Krebs-Henseleit solution) to obtain a steady baseline.
The first hypothesis is a steady baseline state with no rhythmic phenomenon associated with food intake.
Control experiments were first established whereby cells were perfused with KHM buffer until a steady fluorescence baseline had been achieved (Figure 2B(i), CaBP7 and Figure 2B v), CaBP8).
Images were acquired at 4 minute intervals from a population of seven cells until a two-point steady baseline was achieved.
Regeneration of the chip surface was achieved by injection of 20 µl 0.1 M NaOH until steady baseline was achieved.
Briefly, one FM/CMC column was equilibrated with 5 mM ammonium acetate until steady baseline was obtained, and the retention time of TALA (10 mM, injection volume 1 μL) was recorded by TOFMS.
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