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The cells were then seeded at different intervals until a stable expression was observed by microscopy.
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UGT94H1 expression peaked at 8 DAA, declined at 16 DAA, and maintained a relatively stable expression afterwards until maturity.
However, the silencing effect started only after the construct had become stably integrated in the host cell and resulted in a gradual decrease in reporter expression until low but stable expression levels were reached (26).
The effect of LMP1 expression was assessed using both a transient and stable expression system.
The expression pattern of PPARGC1B and ERRA was similar to CYC1, with stable expression until day 4, followed by lower expression at subsequent time points.
FUS3 is encoded by two genes in soybean, each producing one transcript (Glyma16g05480.2 and Glyma19g27336.1) in developing embryos, both genes showing similar and stable expression until day 55 when their levels dropped significantly [ 26].
After leaving 2 s, continuous reading of 10 s Relative Luciferase Units (RLU), and retaining the high RLU value cell clones passages every time subsequently luciferase activity of cells was detected each ten generations and gradually eliminated lower RLU value cloned cell until passaging to the 40th generation; stable expression of the luciferase clone HCT116-luc+ is positive clones.
A lower M value indicates more stable expression.
The aim of our study was to identify a group of genes that show a stable, nonchanging expression profile in immune cells under altered gravity conditions over a time range of seconds until several minutes.
AMISOM's mission was to support transitional authorities until a stable government could be established.
The electrode was left in the sample until a stable reading was obtained.
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