Sentence examples for until a final concentration from inspiring English sources

Purified Rfa2 was concentrated by using centrifugal concentration tubes (Amicon, Millipore) until a final concentration of 0.8 1.0 mg/ml was reached.

For the experiment with stepwise osmotic stress conditions, cells were grown until late exponential phase and then salt-stressed with NaCl added in 2, 5 or 10 steps until a final concentration of 1 M for 24 h at 40 °C.

The test water was prepared by dropping 1 mM Pro into Toya Lake Station water contained in 60-L tanks until a final concentration of 1 µM Pro was reached.

The test water was prepared by dropping 1 mM Pro or Glu into Toya Station water contained in 60-L tanks until a final concentration of 1 µM was reached.

Oxygen was added until a final concentration of 1%.

For each strain, 100 ml of LB were inoculated with 10 cells/ml and incubated at 37°C until a final concentration of about 2 × 10 cells/ml.

Then, the blot was incubated with anti-IGF-1 (Upstate Biotechnology, diluted in PBS-milk until it achieved a final concentration 1 μg per ml) overnight at 4°C.

The refolded protein was then concentrated using 30 KDa centricon to a final concentration of 2 µg/µl and stored at 4°C until further use.

DNase-free polyA+ RNA was stored at a final concentration of approximately 100 ng/mL at −80°C until use.

The embryos were then treated with hydroxyurea (H8627; Sigma) at a final concentration of 5 mM from stage 3 until stage 10.

Media was changed and antibodies added every other day at a final concentration of 1 µg/ml for bivalent anti-MUC1* until discrete colonies were visible.