Next day, the transfection medium was replaced with PM for 1 day, followed by G418 (Invitrogen) selection at 2000 μg/ml over several days until a day after all cells in the control untransfected flask had been eliminated by G418.
No one came to help us until a day later".
She knew nothing about this until a day later".
Sometimes, the severity of such a hit, and its lingering effects, are not known until a day or two after.
Unlike produce grown far away and delivered to supermarkets, many of the farm's crops remain in the ground until a day or two before members get them.
And Mr. Watters, the security consultant, said people who planned functions for expatriates frequently kept the location secret until a day before the event.
The messages, in Arabic, were not translated until a day after the attacks, and said: "The match is about to begin" and "Tomorrow is zero hour".
I love an arbitrary target as much as the next runner, but I hadn't even known sub-5 was a thing until a day before.
He knew nothing about hockey until a day in 1938 when he noticed a newspaper headline about the Rangers while riding on a Brooklyn trolley.
Her worries gnawed at her until a day in April 1999, when she had a fight with her mother, Violet Logrono, a real estate agent, about money.
"Until a day ago, Michigan was one of the states where Jill Stein was throwing her temper tantrum," McDaniel said at the rally.
