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This paper screens out some unsuitable samples based upon the following principles.
mRNA quality was assessed in several representative cases using a Bioanalyzer (Agilent Technologies, Japan) to exclude unsuitable samples.
Many genotypes were missing, mainly for controls because of difficulties in extracting DNA from hair samples (n = 117) and for a few cases because of unsuitable samples (n = 13).
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At each interval the specific subsample location was selected manually by moving the stage no more than 20 μm laterally from the transect line to avoid unsuitable sample locations (i.e., conceptacles or reproductive structures and uncalcified cell interiors)19.
The diagnosis was confirmed by phenotyping for 13 of these patients (36.1%) but was not confirmed by phenotypic typing for the remaining 23 patients due to unsuitable sample type (virus inactivated) or negative culture results.
Moreover, these nanometer-sized metal oxides are not target-selective and are unsuitable for samples with complicated matrices.
Removal shall not be permitted if there is reason to believe that the data sought cannot be obtained from such a sample (e.g., the technique is not sufficiently well known to consistently produce satisfactory results) or the specimen is known to be unsuitable for sampling (e.g. old or treated with heat or chemicals inimical to DNA sampling).
Different countries and institutes have developed a variety of gear types, but these are often unsuitable for sampling rough seafloor, such as seamount and ridge topography.
For example, the EP-SM kit performs the centrifugation at 1,000 2,000 g in some cases, which may be fair for soils (the density is much higher than activated sludge), but unsuitable for sludge samples, and could cause loss of sample.
In addition, this technique may be unsuitable for CFA samples containing a high carbonaceous fraction and a low concentration of V because leaching V may not be efficient.
Thus, field samples unsuitable for full-genome sequencing may be amenable to microarray analysis.
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