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At each interval the specific subsample location was selected manually by moving the stage no more than 20 μm laterally from the transect line to avoid unsuitable sample locations (i.e., conceptacles or reproductive structures and uncalcified cell interiors)19.
The diagnosis was confirmed by phenotyping for 13 of these patients (36.1%) but was not confirmed by phenotypic typing for the remaining 23 patients due to unsuitable sample type (virus inactivated) or negative culture results.
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This paper screens out some unsuitable samples based upon the following principles.
mRNA quality was assessed in several representative cases using a Bioanalyzer (Agilent Technologies, Japan) to exclude unsuitable samples.
Many genotypes were missing, mainly for controls because of difficulties in extracting DNA from hair samples (n = 117) and for a few cases because of unsuitable samples (n = 13).
Different countries and institutes have developed a variety of gear types, but these are often unsuitable for sampling rough seafloor, such as seamount and ridge topography.
Moreover, these nanometer-sized metal oxides are not target-selective and are unsuitable for samples with complicated matrices.
Batches of some buffycoat extracts on filter paper (for DNA) stored in the freezer were soaked with water due to a freezer failure rendering some samples unsuitable for further analysis; the batch of samples of level 6 in Kerr Seringe was particularly affected (Table 2).
Ultrasound microbial cell disrupters operating at around 20 kHz are often physically large and, due to significant heating, can be unsuitable for small sample volumes where biochemical integrity of the extracted product is required.
Automatic techniques have been developed to segment cell nuclei from tissue sections or whole Drosophila brains in 2D and 3D images [12] [15], but they require intensive computation, making them unsuitable for large sample sizes.
This caused irreversible damage and made the sample unsuitable for future studies.
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