Subsequent mutation of Lys165 to a cysteine residue, produced an enzyme with only low activity, as expected, but this mutant enzyme was unsuitable for chemical modification because of its poor long-term solubility, slowly precipitating over a period of 1 2 days.
Modification of unsuitable soil types with lime or even industrial waste material presents a further option [24, 25], although any potential material should be tested in laboratory conditions before use as barrier.
Higher throughput proteomic approaches may solve this problem, although they are often unsuitable for quantitation of posttranslational modifications such as phosphorylation.
Basanta-Sanchez et al. (2016) demonstrated a highly sensitive, accurate, quantitative UPLC-MS/MS nucleosides analysis method; however, this method applies to cellular RNA modifications and is thus unsuitable for human biological samples, such as blood and urine, due to its narrow calibration range.
Although these reports firmly establish the strong effect of age on epigenetic modifications, individual CpG sites are unsuitable for global contrasting of the epigenetic ages of different tissues derived from the same individual.
The remaining histone modifications available on ENCODE are unsuitable for this study as they either target non-promoter regions (e.g. H3K36me3 in the 3′-UTR [ 13]) or are mutually exclusive and thus highly redundant with those selected for this study (e.g. H3K9/27 ac).
It is known that ethylene is the hormone that controls the ripening of climacteric fruits, such as peach [ 44], which makes it an unsuitable candidate for specific aroma control since its modification could alter the whole ripening syndrome.
In spite of the sufficient performance with static image analysis, these methodologies usually result in low frame rate and therefore are unsuitable for tracking with embedded video systems without any modification (Fotouhi et al. [2009]).
Although these modifications, to some extent, have proved successful they would be unsuitable as a method of expanding cells for use in cell-based repair therapies and, as such, monolayer culture is a limiting factor for chondrocyte efficacy.
However, problems such as low protein expression level, lack of accurate post-translational modifications as well as complex purification procedures have made current approaches unsuitable for large-scale production.
However, genetic modification using episomally maintained plasmids has two limitations that make it unsuitable for a number of parasite transformation studies.
