Presumably, the treatment for 24 h may be unsuitable for the expression of some disease resistance genes.
However, several reports demonstrated that the transcript levels of these genes also vary considerably under different experimental conditions and are consequently unsuitable for gene expression studies [ 6, 11].
The rapid loss of GFP in soybean cotyledonary cells made analysis difficult and this target tissue is completely unsuitable for transient expression analysis using single time point determinations.
The fact that the GAPDH partitions the Uppsala samples into good and poor prognostic groups is added evidence that it is not only unsuitable for scaling expression values, but may in fact be a candidate oncogene.
Although this is a worthwhile longer term aim, the state of the art is that existing ontologies are not orthogonal or interoperable, and many present a focus that is unsuitable for gene expression data.
Using a parallel analysis of the tissues by immunohistochemistry, we also identified orthopedic biopsies that were unsuitable for gene expression analysis of synovial inflammation due to sampling of non-inflamed parts of the tissue.
The Shine-Dalgarno sequence carried by pME6032 was removed, as it is unsuitable for the expression of a non-coding sRNA gene, by a second round of PCR using the primers 6032K (HpaI) and 1698Sur6 (PstI) (Additional file 3), digestion by HpaI and PstI, and cloning.
Or it might be unsuitable since various expressions in it are not precise enough, too context sensitive, or in other ways not ideally suited for the philosophical project.
But some are still instinctive or unsuitable with the expressions defined, such as the viscosity and porosity, with less consideration on the impacting factors and function mechanisms in low-frequency vibration production technology.
Hence, in our hands commercially available antibodies were unsuitable to study RGS2 expression in native tissues.
In fact, all other vectors tested to date have proven to be unsuitable for high-level transient expression: Hairy roots infected with the TRV vector expressed at least an order of magnitude more GUS than the highest level previously reported with the replicating pBY031-I1 vector derived from Bean yellow dwarf virus.
