Because γ-TuSCSpc98Δ1 177 formed aggregates already in TB150 buffer and was therefore unsuitable for oligomerization assay, we focus our analysis on spc98 Δ1–156.
Similar to FRET assays, the rapid drop in signal with increasing distance between donor and acceptor molecules makes such assays unsuitable for the detection of protein phosphorylation, even if the proteins could be labeled with appropriate dyes [ 9].
The high frequency of MBL deficiency and functional defects [5], [9] makes MBL mediated complement function assays unsuitable for elucidation of deficiencies downstream of MBL itself.
The antigenemia assay proved unsuitable for the surveillance of hematological transplant patients.
The original assay was unsuitable for the nonproliferating TSQ due to the lack of cellular outgrowth.
In addition, the TT is exquisitely sensitive to the presence of dabigatran, and samples may be unclottable at therapeutic levels, making it unsuitable as a quantitative assay.
Thus while there is no evidence to indicate that the IGS assay is unsuitable for diagnostic work, the use of the ITS assay for this purpose represents the more conservative approach.
The single largest barrier to using circulating DNA for cancer detection is the amount of circulating undegraded DNA that can be isolated is low, making it unsuitable for currently available assay technologies.
The 1X and 2X UAS lines showed weaker expression patterns and were therefore unsuitable for our future assays.
Because of the apparent weak binding for these variants, they were deemed unsuitable for performing transfer assays.
