Sentence examples for unsuccessful expression from inspiring English sources

This highlights a further advantage of autologous expression over the often unsuccessful expression of human genes in E.coli [31].

The unsuccessful expression of these proteins in E. coli is probably due to their toxicity when expressed in bacterial cells.

Successful protein expression was defined for values of 2 to 4 and unsuccessful expression for values of 0 and 1.

Similarly, previous attempts to complement a ΔltnJ mutant of Lactococcus lactis with npnJ were unsuccessful, and insoluble expression could not be ruled out.

The possibility of unsuccessful As-dmtA expression in the transformants was excluded by RT-PCR amplification of mRNA prepared from the As-dmtA introduced mutants (Fig. 4C).

We were unsuccessful in obtaining LipA expression in A. niger (data not shown), but obtained high expression in N. benthamiana in transient co-expression of the lipA gene with the suppressor of posttranscriptional gene silencing p19 [ 12].

Whereas successful complementation of levQ or levS deletions was achieved by introducing a wild-type levQ or levS sequence on plasmid pMSP3535, efforts to clone the levT gene in E. coli in a configuration that would allow for expression were unsuccessful.

However, they were unsuccessful in predicting the expression of two experimentally proven AltORFs, AltPrP and ALEX.

Attempts to restore solvent formation by these acid production mutants by plasmid based adhE expression were unsuccessful for the M5 BKKO strain.

One of the reasons for unsuccessful in vitro protein expression in E. coli may be the presence of secondary structures in mRNA, which may inhibit translation [ 19].

When the domain composition of a target is poorly understood, or if there are insufficient structure data to guide sited directed mutagenesis, long iterative phases of subcloning or mutation and expression often prove unsuccessful despite much effort.