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The complex seems to be designed to expose an unstructured domain of Dsl1p at its top, which is required to capture vesicles.
Furthermore, N1 encompasses the unstructured domain of PrP [10] and is unstable.
Initial examination of the 2D 1H,15N-HSQC spectra for p12CAN revealed a large subset of signals with poor chemical shift dispersion in the proton dimension, suggestive of a largely unstructured domain within the protein (Figure 1).
We gratefully thank Dr. Tatyana Kolesnikova for input on immunofluorescence protocols and for assistance identifying salivary gland chromosomal regions, Dr. Sarah Bondos for analyzing the intrinsically unstructured domain potential of SAF-B, and Dr. Kathryn Ryan for the nuclear pore complex antibody.
In this model, the fusion loop/peptide would lie within the poorly structured domain II, and the E2 606–625 peptide would be found in the globally unstructured domain III, where a critical His residue is disposed at the interface with domain I.
Due to the unstructured domain, SmGPxB shows higher similarity to mitochondrial AtGPx6 (data not shown).
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The use of linear triangular elements permits the construction of accurate (by virtue of the second-order spatial and temporal accuracies of the scheme) and efficient (by virtue of the less stringent CFL condition of Lagrangian methods) schemes on unstructured domains.
Others have noted the preponderance of intrinsically unstructured domains in chromatin remodeling proteins and transcription factors, which may play a role in nuclear structure[39].
The proposed methods respectively employed domain-specific vocabularies, a manually annotated corpus and unstructured domain-specific documents.
One of the features analyzed, was the presence of SNPs in natively unstructured domains.
Based on predictions made using IUPred [ 41] we identified globular and natively unstructured domains in T. cruzi proteins (globular regions ranged from 10to90%0% of the protein).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com