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The second method has the problem of unstable expression in E. coli, mainly because the exogenous protein is expressed in inclusion bodies and so requires renaturation of the inclusion body protein.
By the sixties, its edicts, as set out by early practitioners like Chuck Berry, Jerry Lee Lewis, Fats Domino, and Little Richard, were decreed in full: rock and roll should be scrappy and instinctive, a wild and unstable expression that appears free of mediation or meddling, even (especially) when it's not.
Production of these often complex transgenic proteins is achieved predominantly in mammalian cell lines but the process is hampered by low yields and unstable expression.
Even if many previously established T cell lines could overcome this restriction after transduction of the expression vectors (Wu et al., 2002; Krowicka et al., 2008), the random integration in these lines leads to the unstable expression of transgene and also could affect the expression of the nearby genes (Modlich et al., 2005; Nienhuis et al., 2006).
These results were consistent with the earlier reports, which found ACTB to be highly regulated by treatments and have unstable expression in hypoxia-cultured human chondrocytes and lipopolysaccharide-stimulated human THP-1 and K562 cells (Cao et al. 2012; Foldager et al. 2009), whereas B2M was the least stable gene in human reticulocytes (Silver et al. 2006).
The transformant rickettsiae had low level and/or unstable expression of the foreign gene.
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Our task is to distinguish the genes that show stable expressions from those show unstable expressions in the unstable pathway.
The wt SCD was unstable upon expression, whereas expression of SCD K/A resulted in the stabilization of the protein.
However, since typical experiments use on the order of k ≈ 30 PCR cycles, analytic derivatives of P with respect to T result in unwieldily and numerically-unstable expressions.
To finely characterize this unstable reporter expression in AIZ, we first tried to develop truncated promoter/enhancers to specify the expression in AIZ.
Importantly, the introduction of chimeric TCR bypasses problems associated with the introduction of nonmodified TCR genes, such as pairing of introduced TCR chains with endogenous TCR chains and unstable TCRα expression.
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