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We don't educate, train and evaluate airline pilots, surgeons, electricians or plumbers with unspecific standards.
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An antibody responder was defined as a subject who had an anti-nicotine IgG titer, which was larger than the unspecific background reactivity (average plus three standard deviations) of the ELISA.
Bearing in mind that the use of ionic strength is a standard approach to neglect unspecific interactions and that S-transnitrosylation by GSNO implies the specific interaction between GSNO and the target protein, it is reasonable to assume that these specific interactions are stronger and require a higher ionic strength to be disrupted.
Consequently the measure is unspecific.
For the LC-MS/MS spectra at 1 mg/L, we found similar results compared to pure standards (i.e., no unspecific MS/MS signals fit the predefined criteria).
Furthermore, the instructions in the highly standardized and widely accepted ISO standards for the identification of microbes in food are usually rather vague and unspecific with respect to the sampling process as well as the sample pretreatment and homogenization (in contrast to the downstream detection procedures).
Signal from wells incubated with CSF and sulfo-Tag anti-mouse Igs (secondary antibodies) did not differ from zero standard indicating no antibody-related unspecific binding.
Here, we present a method for selection and enrichment of beads with correct products on the surface, thus removing beads carrying unspecific sequences, which were not removed by standard gel purification protocols.
However, ten years after initial diagnosis the patient developed unspecific neurological symptoms that could not be classified by standard neurological diagnostic work-up.
Interestingly, despite thorough purification by gel electrophoresis, short unspecific fragments still accounted for a large proportion of the standard-enriched control sample library.
Two lymphomas demonstrated unspecific PCR inhibition indicated by a suppression of the internal amplification standard.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com