This trend change may be explained by the assumed unspecific high number of false-positive miRNA target site predictions that can be partly filtered out by filtering by their RNA duplex energy values [11], [14], [30].
The method of non-covalent immobilization of DNA probes on an uncharged, non-reactive, hydrophilic support lowers the unspecific binding and the number of handling steps required to conduct the experiments for the detection of DNA on microarrays.
This is challenging due to the vast number of unspecific fragments sequenced along with the ChIP-enriched ones.
A temperature increase above 60°C reduces the number of unspecific spots but leads to the overall decrease of fluorescence, complicating the analysis and negatively impacting the overall performance of the test.
as well as a number of unspecific factors.
Similarly, the CD66b+ cells have the highest number of unspecific transcripts, whereas CD8+ cells express the least (data not shown).
When additional filtering through other classes (e.g. diagnostic or prognostic methods) is applied, the typical high number of unspecific search results is significantly reduced.
The CFT has a sensitivity of at least 97% [ 14] but a notable number of unspecific, false positive results occur [ 15- 18].
The protein Smr, staphylococcal multidrug resistance pump, binds substrate in isotropic bicelles, but shows only reduced or unspecific binding in a number of detergent systems.
However, underproliferation is the most common phenotype in our analysis (538 of 620 genes) and can also arise from a number of unspecific biological defects, such as cell death or cell-cycle block.
