For the characterization of the generated amplicons and to control the contamination by unspecific by-products, a melting curve analysis was applied.
A melting curve analysis was performed at the end of each run to rule out contamination with unspecific by-products that affect the quantitation of the PCR product.
The DNA was either isolated from a gel (in case of unspecific by-products) using the QIAquick Gel Extraction Kit (Qiagen) or cleaned with ExoSAP-IT (USB) according to manufacturer's instructions.
The amplified DNA was either isolated from a gel (in case of unspecific by-products) using the QIAquick Gel Extraction Kit (Qiagen) or cleaned with ExoSAP-IT (USB) according to manufacturer's instructions.
In addition, unspecific coimmunoprecipitation by the anti-FLAG-Sepharose could be excluded by mock immunoprecipitation with normal A-Sepharose (Figure 1A).
Vincent Bataillon's screen direction of Sunday's presentation of the May 12th performance heightened the unspecific feeling by taking an exceptionally remote view of the action.
PCR amplification of the simulant with virus-specific primers revealed products of the predicted length, in bands of similar intensity, and without detectable unspecific products by electrophoresis analysis.
The results indicated that DSBs triggering deletions in lymphoid leukemia are formed at a few defined sites by illegitimate action of the RAG protein complex, while DSBs in solid tumors are formed at unspecific sites by factors unidentified yet.
It is known that the imaging quality of a tracer could be improved by increasing its hydrophilicity, which leads to a reduced plasma protein binding and diminished the unspecific uptake by various organs.
