We compared the proliferation kinetics of endothelial cells from atrium unsorted, atrium sorted, ventricle unsorted, and ventricle sorted cells.
The comparison of unsorted and sorted cultured populations revealed enrichment of endothelial cell markers in FACS-sorted atrium EC compared to unsorted atrium cells such as Flk-1, Flt-1, Tie-2, vWF, VE-Cad, and CD31.
Unsorted, CD90Hi-sorted, and CD90Lo-sorted murine ADSCs were reprogrammed using standard 4F transduction.
The reprogramming efficiencies of unsorted, CD90Hi-sorted, and CD90Lo-sorted ADSCs were 100%, 116.5%, and 74.7%, respectively.
The relative reprogramming efficiencies of unsorted, CD90Hi-sorted, and CD90Lo-sorted ADSCs were 100%, 116.5%, and 74.7%, respectively.
The trend in the conductance in unsorted and sorted samples is confirmed by current-voltage measurements.
Similar to neurons in unsorted cultures, sorted neurons were electrophysiologically active.
Unsorted and sorted beads were amplified with Phusion DNA polymerase (Finnzymes, Espoo, Finland) using a single-molecule PCR protocol described previously [25].
To validate the enrichment, the unsorted and sorted cell fractions obtained by FACS was analyzed under fluorescence microscopy, or fixed, embedded, sectioned, and stained as above to determine the percentage of germ cell type.
In order to confirm host shutoff activity in the samples prior to library construction, RNA isolated from unsorted and sorted cells was analyzed for GAPDH mRNA levels by quantitative real-time PCR (qPCR; Fig. 1C).
