For separation of soluble StyB from unsoluble matter, the suspension was centrifuged for 2 h.
Concerning Claim (iv), finitely presented soluble groups with unsoluble word problems have been constructed by Kharlampovich in [102] and by Baumslag, Gildenhuys and Strebel in [24].
El-Mufleh et al. (2013) showed that PAH are mainly sequestered in the unsoluble part of sediment organic matter (humin) [6].
Unsoluble material was separated by centrifugation (20,000 g, 40 min).
Human glomerular samples were denatured by boiling in Lämmli buffer for 5 minutes, and unsoluble components pelleted by centrifugation.
For soluble and unsoluble samples, 10 μl were used for dot-blotting, and 5 μl for PAGE-electrophoresis (see "Immunoblotting" section).
The water unsoluble property of CXCL10 protein limits its isolation, purification, and crystal growth, so it was difficult to determine the structure of CXCL10 protein.
Unsoluble material was removed by centrifugation at 12,000 × g for 20 min at 4°C and aliquots were used for protein determination according to Bradford (1976).
Each sample was then centrifuged at 20,000 g, the unsoluble fraction was washed twice, and resuspended in 50 μl of 50 mM TrisHCl, 150 mM NaCl, and 3 mM NaN3 (pH 7.4).
Unsoluble material was removed by centrifugation, and the oligomers were purified on a Superdex75 (GE Life Sciences, Uppsala, Sweden) column (self-packed XK16/100, 180 ml bed volume), at a flow rate of 1 ml/min, and a 1 ml sample collection volume.
Unsoluble material was separated by centrifugation, and the clear solution was immediately loaded on a Superdex75 (GE Life Sciences) gel filtration column (self-packed XK16/100, 180 ml bed volume), with a flow rate of 1 ml/min and a 1 ml sample collection volume.
