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The unsatisfactory conversion of sugar to lactic acid may be ascribed to the low pH during the late fermentation (about 4.0 after 24 h fermentation), which has a severe negative effect on cell growth and metabolism.
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Some remain which, often for reasons of equilibrium limitations, provide unsatisfactory conversions or yields.
We chose not to evaluate the results of the methylation levels obtained from FFPE DNA extracted using the classical method because the number of unsatisfactory conversions was too high, but it was clear that poor bisulfite conversion led to overestimation of methylation (Additional file 3: Figure 1).
The epidural block level should be reassessed and if found to be unsatisfactory, an immediate conversion to general anesthesia should occur.
Good communication with the obstetric team to anticipate a cesarean delivery will allow for adequate planning for safe conversion of unsatisfactory epidural analgesia to adequate surgical anesthesia.
In conclusion, we demonstrated that the use of FFPE tissues induces unsatisfactory and non-reproducible bisulfite conversion leading to unreliable results for methylation levels.
FFPE samples gave unsatisfactory and non reproducible bisulfite conversions leading to random results for methylation levels.
In these existing works [12], [23]-[29], theugh the issue of the watermark against DA/AD conversions has been mentioned, the robustness performance is unsatisfactory.
This year, she received "unsatisfactory".
It is very unsatisfactory".
This is totally unsatisfactory.
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