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Use of unreliable reference genes for normalisation may lead to inaccurate quantitation of miRNAs of interest [ 17, 18].
Although being extremely powerful technique, qPCR suffers from certain pitfalls, noteworthy the use of unreliable reference genes for the normalization step [ 5].
Use of an unreliable reference gene for qRT-PCR analysis may contribute more to the noted inconsistency than has previously been estimated.
Consequently, the transfers between close relatives will only rarely affect the relative placement of families and higher taxonomic units; however, the scaffold may be an unreliable reference for within family and within-genera phylogenies.
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However, utility depths are rarely referenced to a recognized elevation datum (Federal Highway Administration (FHWA) 1999 Anspach 1995) and any changes of the reference surface make the buried depth a very unreliable source for deriving vertical utility locations.
He has also displayed flashes of pragmatism: according to a cable published by WikiLeaks, Mr. Li confided to the American ambassador that he considered China's economic figures unreliable and "for reference only".
When using conventional femoral guidance jigs, deficiencies of the lateral femoral condyle often render the posterior condylar axis unreliable as a reference to determine femoral component rotation, resulting in an inadequately rotated femoral component.
And for all the "smoke-and-mirrors" techniques utilised by the show's creator Nic Pizzolatto – obscure literary references, unreliable narrators and the like – the dissolution of Cohle and Hart's partnership is as uncomplicated as it gets: two men beating the hell out of each other in a car park after one found out that the other had slept with his wife.
Teasing references to unreliable narrators.
In such case, the estimation obtained from the reference is considered unreliable, and block warping is only performed with the remaining reference frame.
But as beautiful as Malek's speech was, it would be difficult to top his opening line which saw him looking at the audience in awe and making direct reference to the unreliable narrator he had just won an Emmy for portraying.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com