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A CDF of distances from unrelated expression vectors provides an unbiased worst-case distribution and the baseline CDF is plotted in Figure 2. By comparison, if two platforms agreed completely, the CDF would be a unit step function.
One possible explanation for the maintaining of the clusters is the sharing of regulative elements [ 99], however, our expression data do not support this theory since genes from the same cluster show partially unrelated expression.
The relationship between these two mechanisms also sheds light on the importance of selective pressure in the evolutionary process; random drift under a neutral model would result in unrelated expression and sequence changes, while strong selective pressure would be reflected by a positive correlation.
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These findings suggest that the antiproliferative effects of some antisense oligonucleotides may be unrelated to expression of the gene they have been designed to target.
Furthermore, we show that (1) DNA copy number at the EEF1A2 locus is unrelated to expression level, (2) there are no activating mutations in the eEF1A2 coding sequence in tumours where there is overexpression in the absence of gene amplification and (3) the methylation status of the EEF1A2 gene is unrelated to expression level.
Given the fact that the HRC algorithm is insensitive to the settings in the above cross validation tests, we are confident that given a particular gene slide of interest the HRC metric is able to help us filter out a large number of unrelated gene expression images without human intervention.
A fragment of about 60 kDa was detected in all HEK 293-T cells, including untransfected and YFP-reporter only expressing controls (Figure 6A), indicating that this fragment is unrelated to YFP expression and is recognized by AB-290 non-specifically.
However, in our study, CpG 113 (−93) and 114 (−81) were hypomethylated in all cell lines and unrelated to the expression of MUC4 gene, whereas sites 108 and 109 were associated with MUC4 gene expression.
A normal pattern of PERP membrane staining was observed in most AEC patients, although in two unrelated patients PERP expression was reduced in the basal and suprabasal layers of the skin (68), consistent with either reduced PERP expression or with protein delocalization due to alterations of other desmosomal components.
Array comparative genomic hybridization (CGH) analysis was also employed in an effort to identify gene expression unrelated to HRG treatment.
The reduction of Tf uptake in pOPTNWT-GFP-transfected cells was thus unrelated to the expression level of total TfR.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com