PubMed, Cochrane Library, and CINAHL were searched for relevant studies and grey literature was searched to retrieve unpublished methods.
A limitation of a literature review is that unpublished methods, if any, are omitted from this review.
R191A and F196A mutations in the PPIase active site were introduced by site-directed mutagenesis in the CYP33 coding sequence to create a PPIase dead CYP33 (P. Breslin, unpublished methods).
An investigative commission set up in January by Erasmus reviewed Simonsohn's unpublished method with the help of two outside statisticians and concluded it was valid.
First, using an unpublished method of Lindenbaum explained to him by Tarski which holds for systems that have the rule of Substitution for propositional variables, McKinsey shows that there is an S2-characteristic matrix M = (K, D, −, *, ×) that does not satisfy condition (iii) and is therefore non-normal.
HRV positive samples from hospitalized and control patients during September 1, 2004 through August 31, 2005 were further identified to species by RT-PCR and sequencing of partial region of the VP1 (D. Erdman, unpublished, method available on request).
An unpublished method (http://huguenard-lab.stanford.edu/public/) noted in [5] could detect photostimulation-evoked EPSCs based upon the estimated EPSC differentiation window sizes and event amplitudes, which need to be carefully adjusted and tested on recorded traces of each map to ensure detection of synaptic events.
Quinine drug analysis was performed using LC with fluorimetric detection (unpublished method).
Gene expression profiles were analyzed using a method called "extracting microarray gene expression patterns and identifying biologically significant genes" (EPIG; Chou JW, Zhou T, Paules RS, Kaufmann WK, Bushel PR, unpublished method).
We previously developed an RNAi delivery assay based on feeding recently emerged larvae with detached tomato leaflets that had absorbed aqueous solution containing in vitro transcribed dsRNA of the target genes (Camargo et al., unpublished method).
Together with the novel candidates, the experiment included three other target genes (V-ATPase, AK, EcR) that were previously evaluated by our group in T. absoluta (Camargo et al., unpublished method), together with a negative control (dsRNA specific for GFP).
