The trial statistician for Mori 2015 provided unpublished, cluster-adjusted odds ratios for our use.
We included seven RCTs that enrolled 17,505 participants; two individually RCTs (Ansah 2010 GHA; Bisoffi 2009 BFA), two published cluster-RCTs (Skarbinski 2009 KEN; Yeboah-Antwi 2010 ZAM), and three unpublished cluster-RCTs (Hopkins 2008 UGA (V High); Hopkins 2008 UGA (High); Hopkins 2008 UGA (Medium)).
For clustering, ESTs were evaluated with MALIGN, a k-mer based alignment tool (Chapman, Unpublished), which clusters ESTs based on sequence overlap (k-mer = 16, seed length requirement = 32, alignment ID ≥ 98%).
When these sRNAs were mapped onto the melon chloroplast genome (unpublished data), two clusters of sequences resolved in regions presumably annotated as chloroplast rRNA.
We used a completely new algorithm (Shahbazkia & Christen, unpublished) that directly clusters tag sequences having less than k differences (k = 0, 1....10) and does not rely on a multiple sequence alignment.
Based on these methods, the twin siblings analyzed in this study, including those who were both diagnosed as autistic, each fall into different phenotypic clusters (unpublished data), with the exception of one set of twins who were discordant in the diagnosis of autism.
One further trial (of 2660 children in Vietnam from 1999) remains unpublished (Hall 2006 (Cluster)).
Six strains from this study belonged to clade 7502 in which in previous studies the majority of strains from human immunodeficiency virus-infected patients clustered (unpublished thesis, Schneider, Bilthoven, The Netherlands).
A second putative exopolysaccharide biosynthesis cluster named EPS cluster 1 (Bbr_1786-1801, unpublished data), was also shown to be present in the other analysed B. breve genomes and it appears to be more conserved than EPS cluster 2 among the strains analyzed (Additional file 6: Figure S5, panel b).
The 9 332 571 short reads were mapped using glint software (Faraut T. and Courcelle E.; http://lipm-bioinfo.toulouse.inra.fr/download/glint/, unpublished) onto the EST clusters generated from the 454 sequencing data and counted per cluster and per library.
However, the whole-genome sequencing of an increasing number of organisms has revealed small, cysteine-rich "hypothetical" proteins that contain CCC clusters (our unpublished observations), which are potentially good candidates for novel MTs.
