We have attempted this approach, and while we have been successful in getting Mps1 and Polo to co-IP under hypoxic conditions (WDG, NMS and RSH, unpublished data), attempts to wash the precipitates sufficiently for proteomic analysis were unsuccessful.
Preliminary work on the bacterium suggested the possibility of inducible prophages in M. catarrhalis strains (unpublished), however, attempts to induce and isolate M. catarrhalis phages were unsuccessful, leading to the choice of a bioinformatics approach to identifying M. catarrhalis prophages.
If a paper had been written from an abstract but was unpublished, we attempted to obtain it from the authors.
21 Finally, an unpublished report, attempting to revalidate the QRISK model on the THIN cohort, failed to replicate the results of the external validation and has further contributed doubts about the performance of QRISK.
On a much larger scale, Feng and co-workers (unpublished data) attempted to account for the rather sizable contribution of mercury to the environment from a variety of activities in Guizhou, a 170,000 km area of China that contains > 60% of that nation's mercury resources.
Third, our recent attempts (unpublished data) and those of Pornwiroon et al. [80] to amplify pRFδ in a different strain of R. felis (str. LSU) were unsuccessful.
This function is obviously not the time keeping by melatonin synthesis as it is in vertebrates, since there is no circadian rhythm in the blAANAT activity; moreover, no melatonin has been found in amphioxus (our unpublished observation), and attempts to identify melatonin receptors in amphioxus were unsuccessful [ 47].
In this study, we searched the xylanase coding regions from the draft genome sequence of A. cellulolyticus (unpublished data) and attempted to clarify the characteristics of the all xylanases (GH11).
DREAM is organized around annual reverse-engineering challenges whereby teams download data sets from recent unpublished research, then attempt to recapitulate some withheld details of the data set.
Furthermore, there is extensive anecdotal and unpublished evidence that many attempts to generate humanized mAbs based on mouse V regions have failed to produce useful Abs because of loss of specificity or affinity.
