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A recent study [ 32] found that UV and fluorimetric determination (average between the PicoGreen and the Hoescht dye methods of determining DNA concentration) agreed quite well when DNA concentration was measured from DNA samples extracted by the CTAB procedure from a relatively simple and unprocessed matrix such as NK603 certified reference material, at different percentages of GM content.
Gel analysis suggests that per each DNA isolation procedure, DNA extracted from the feed, and even more clearly from the biscuit, matrix suffered extensive damage compared to an unprocessed matrix (CRM 1%): the signs of broad degradation are quite striking, with DNA fragments dispersed everywhere between high and very low molecular weight.
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In the case of soybean grain we did not expect a reduced PCR efficiency since the matrix is unprocessed, however the lowest efficiency was obtained for this matrix with the lectin amplicon.
Therefore, a DNA walking approach, corresponding better to the need of enforcement laboratories, has been developed and validated on unprocessed and processed food matrices containing minute amounts of GM targets.
This technology is suitable for both unprocessed and processed food/feed matrices since amplicons of around 100 bp are usually amplified.
These had been chosen to represent an unprocessed material (CRM 1%), a raw mixed matrix (feed) and a processed mixed matrix (biscuit).
MIC-1 is often secreted in an unprocessed propeptide form that regulates the balance between extracellular matrix stores and circulating mature MIC-1 (Bauskin et al, 2005).
The new atomic-level information of the biofilm matrix of V. cholerae has provided a molecular fingerprint in a largely unprocessed state allowing the assignment of the carbon pool to sugar, lipid and protein pools thereby conclusively demonstrating that, unlike the rugose E. coli biofilm (McCrate et al. 2013), the majority of the biofilm matrix is in carbohydrate form (Reichhardt et al. 2014).
The qualitative natures of the spectra for unprocessed HAM and DHAM are alike with little shift in the peaks due to difference in the matrix nature (Fig. 2).
The unprocessed HAM showed broad Tg peak with more heat absorption, reflecting the complex molecular nature of the matrix compared to DHAM.
The marines' accounts were irregular, unprocessed, conflicting.
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