Here we investigate genomic imprinting in the cerebellum using a newly developed Bayesian statistical model that provides unprecedented transcript-level resolution.
SAGE offers an unbiased and comprehensive approach to expression profiling, limited only by the depth of sequencing chosen by the researcher, and offers an unprecedented opportunity for transcript discovery.
RNA-seq provides unprecedented power for transcript and isoform discovery.
RNA-seq gives an unprecedented quantitative measure of transcript levels, and very low technical variability [24], [25].
On Friday, the court released transcripts providing unprecedented insight into the Bo family's inner workings and business transactions.
This difference from previous transcriptomes certainly relies on the library normalization protocol used here but not in previous work, allowing an unprecedented coverage of lower abundance transcripts.
This is in line with transcriptome-wide studies in human, mouse and other species have also discovered unprecedented high numbers of novel transcripts, a large fraction of which were ncRNAs (e.g., [ 4, 5, 7, 25, 50]).
Recent developments of sequencing technologies enable genome-wide identification of fusion transcripts at an unprecedented level of resolution, but these technologies are yet limited by the number of samples that can be analyzed within a reasonable timeframe and at an acceptable cost.
Summary: RNA-Seq is an exciting methodology that leverages the power of high-throughput sequencing to measure RNA transcript counts at an unprecedented accuracy.
In order to systematically identify and characterize bidirectional promoters associated with long non-coding antisense transcription, we took advantage of strand-specific RNA-seq experiments, which provide an unprecedented opportunity to analyze and categorize transcripts [ 22].
