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The inhibiting strain was point inoculated in the center of a Petri plate containing YMEA, and after three weeks the second strain was inoculated to the entire unoccupied plate area by applying 200 µl of autoclaved water containing 800 1200 cells/µl.
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Earlier this season, many of the $2,500-a-game seats behind home plate sat unoccupied, a sight that caused the Yankees considerable embarrassment.
The coating solution was aspirated off, and unoccupied binding sites on the plates were blocked with 2%% bovine serum albumin in PBS at 37 °C for 1 h.
Unoccupied sites on the surface of the assay plate were blocked via application of a solution of 1% bovine serum albumin (weight by volume) for 1 h.
The unoccupied plastic spaces in a 96-well microtiter plate containing immobilized IPEC-J2 was blocked by adding 300 μl/well of a 5%% solution of BSA in PBS.
Unoccupied sites on the surface of the assay plate were blocked via application of a solution of 1% bovine serum albumin (weight by volume) in PBST overnight at 4 °C.
For two hours on Wednesday, Times Square morphed into a militarized zone as the police swarmed an unoccupied van with blacked out windows and no license plates parked in an area set aside for Thursday's New Year's Eve celebration.
As depicted in Figure 2, the strategy of our OGT assay involves five steps starting with attachment of the 6 × His-tagged OGT substrate to the plate through Ni-NTA chelation, followed by the blocking of unoccupied nickel ion.
It, too, was unoccupied.
It was unoccupied.
Entire sections were unoccupied.
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