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As a result, gel purification of the full-length linear miRNA donor DNA is unnecessary (discussed in greater detail in supplementary materials; Additional Files 1, 2 and 3).
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Although the continued survival of threatened populations is so basic a criterion that it may seem unnecessary to discuss, it is indeed a major stumbling block for a successful CMMB program.
Entries included 'she does not want to talk about the future and what may happen', 'does not want to ask any more questions' and 'feels it unnecessary to discuss condition'.
Marriage was discussed yet seemed unnecessary; we're bound by something much deeper, and we didn't need a man-made endorsement to prove our commitment.
In the case discussed, these might include avoiding unnecessary future intervention on the clay slopes.
It will not be discussed in this paper to avoid unnecessary repetition.
This was "unnecessary, frustrating and unacceptable" as the issue had been discussed and settled in budget talks.
However, there were some situations of unnecessary communication present in the system, as will be discussed below.
Mr. Kuroda said that the board had discussed such measures, but ultimately decided fresh steps were unnecessary for the time being.
When working with the theme ugly, students discussed critically the rationality in educational activities, and making unnecessary goods, even litter into an assignment.
Let us further discuss why unnecessary normalization decreases the diversity of solutions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com