Sentence examples for unnatural substrate from inspiring English sources
Thus, this is the first report of a thermostable self-sufficient cytochrome P450 and the engineering of a thermophilic cytochrome P450 for the oxidation of an unnatural substrate.
Furthermore, several mutants of the CYP175A1 domain of 175RF were prepared and one mutant (Q67G/Y68I) catalyzed the hydroxylation of an unnatural substrate, testosterone.
This observation underscores that unnatural substrate tolerance in d-alanine metabolism can be exploited to monitor PG biosynthesis.
Both hydratases are also able to convert the unnatural substrate geranylgeraniol (C20 substrate), which functionally resembles the natural substrate lycopene.
Mechanism-based inhibition has been characterized in TPSs with unnatural substrate analogues such as vinyl-farnesyl diphosphate.
The ability of GDP-FucAz to label fucosylated glycans implies that this unnatural substrate enters the Golgi lumen and is utilized by fucosyltransferase enzymes.
From the synthetic chemist's point of view, the asymmetric oxygenation reaction of unnatural substrates has a tremendous potential.
In the second part of the review we have discussed on the LOX biocatalysis of unnatural substrates mimicking natural substrates, lipoxygenase biocatalysis in non-conventional media, the use of immobilized LOX and biocatalysis with engineered lipoxygenase.
The field of directed evolution has progressed to the point where it is feasible to engineer enzymes for unnatural substrates and reactions with catalytic efficiencies and regio-specificity or stereo-specificity that rival those of natural enzymes.
In the recent past, DNA polymerases optimized for diverse PCR and sequencing applications as well as enzymes that accept a variety of unnatural substrates for the synthesis and reverse transcription of modified nucleic acids have been developed.
Here, this paper reviews the use of family profile analysis to find the correlation between the type of ATs and their substrate specificities, the relation between the 3-D structures of ATs and their substrate specificities, and enzyme engineering for the synthesis of unnatural substrates.
