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The unmodified data are considered as duplicate data since these do not have to be re-written.
Fig. 2 Shown below are unmodified data and decayed gamma distribution convolution fit results for three patients their left thyroid lobes Ⓛ, right thyroid lobes Ⓡ, and the total summed kilo counts per min for the whole thyroid glands Ⓣ.
Our approach is based on the use of real microarray data in which controlled fold changes are introduced into 20% of the data to provide a metric for comparison with the unmodified data.
Independent Western blot experiments, performed by using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control, showed that the actin level was also kept unmodified (data not shown).
Importantly, the random permutation of class labels sharply reduces the apparent accuracy of the classification algorithm for most datasets, indicating that the classifiers derived on original, unmodified data are statistically significant, corresponding to true molecular separation of the two phenotypes rather than being a product of chance.
For all three disorders, the genes that were predicted in the unmodified data set and were reported in Table 2 (blue bars) also appeared in more than 50% of the cross-validation samples, and the majority of these genes was encountered more than 75% of the time.
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In this experiment, we utilized the ability of Pol X to incorporate 2′,3′-dideoxyguanosine 5′-triphosphate (ddGTP) with an efficiency similar to that for unmodified dGTP (data not shown).
Unmodified volume data, such as computed tomography or magnetic resonance images, can be rendered directly with this approach, making it particularly suitable for applications in medical or surgical simulation.
Imaging analysis by electron microscopy showed that the pVII modified virions were morphologically identical to the unmodified virions (data not shown), ruling out that the pVII modification introduces any gross physical alterations of the virions as compared to their unmodified counterparts.
The analogue retained cytotoxic potency within 10 fold of unmodified TP201565 (data not shown).
No significant changes were observed for H3K9me3, or H4K12-Ac, H2AZ, and unmodified H3 (data not shown).
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