Sentence examples for unlysed cells from inspiring English sources

The samples were centrifuged at maximum speed for 5 min to remove unlysed cells, and the supernatants were collected, mixed with SDS-PAGE sample buffer, and subjected to western blot analysis with anti-JNK and anti-actin antibodies (loading control).

Unlysed cells and debris were cleared from the lysate by centrifugation for 5 min at 3,000×g.

Extracts were spun for 30 min at 100,000 g to remove unlysed cells and insoluble material.

The cell homogenate was centrifuged for 2 min at 800×g to clear the lysates from cellular debris and unlysed cells.

Cell pellets were disrupted using the FastPrep FP101 bead beater (Bio 101) and centrifuged to remove unlysed cells and cell debris.

The aqueous phase was transferred to a new tube and centrifuged at ∼8000 x g for 2 minutes to remove cell debris and unlysed cells.

After removal of unlysed cell remnants and nuclei by centrifugation in the Eppendorf micro-centrifuge (5 min, 10,000 rpm, 10,200×g); protein concentration was determined by standard method, as described [27]; and 10 μg of equal amounts of protein was loaded into 15%% polyacrylamide gel.

After removing unlysed cell components by centrifugation (6000  g, 10 min, 4 °C) protein concentration of lysates was assessed using the Bradford protein assay (Bio-Rad, Vienna, Austria).

The homogenate was centrifuged at 200  g for 10 min to removed nuclei and unlysed cell, and the resulting supernatant was centrifuged at 100 000× g for 1 h.

After removal of unlysed cell remnants and nuclei by centrifugation in an Eppendorf micro-centrifuge (5 min, 10,000 rpm), the protein content of the supernatant was determined by the Bio-Rad protein assay (Biorad Laboratories, Hercules, CA, USA).

After 15 min at 37°C, the unlysed blood cells were pelleted by centrifugation (5500× g, room temperature, 1 min).