Proceed as previously indicated.
For the survival analyses, the clinicopathological parameters were dichotomised as described previously unless indicated elsewhere.
All oligonucleotide primers are listed in Table S2 Competent E. coli [30] and C. perfringens [31] cells were prepared and transformed as described previously, unless otherwise indicated.
Parents will be sent a summary of the final results of the study unless they have indicated previously that they do not wish to receive such a summary.
Both pieces of in-house software used for SAXS data analysis, 'saxs_multiavg.py' and 'saxs_combine.py', have been deposited at GitHub.com (https://github.com/agardd/saxs_codes). Steady-state kinetic measurements for various Hsp90 homolog and mutants were carried out in previously described conditions unless otherwise indicated (Lavery et al., 2014).
For reference, all measurements associated with WT Kv4.3 were acquired previously [9], [10], [16], [25], unless otherwise indicated.
Most experiments were initiated 24 hrs after transfection, unless otherwise indicated and performed as described previously [48].
Yeast cells were grown at 25 °C in media prepared as previously described in [ 23], unless otherwise indicated.
Study designs have been previously reported [ 18- 20] and were similar, unless otherwise indicated.
Unless otherwise indicated, all other chemicals and reagents were obtained from previously described sources (Mironov et al., 2001).
Unless otherwise indicated, experiments were performed in HEK293T cells, which were cultured as previously described (66).
