Fold change indicates mRNA expression levels in gut and carcass, comparing wild type versus nub mutant, data taken from the microarray data in Additional files 5 and 8 unless other wise indicated.
In relation to children it offers the following guidance "Genetic tests in respect of children when, according to applicable law, that child does not have capacity to consent should normally be deferred until the attainment of such capacity, unless other factors indicate that testing during childhood is clinically indicated.
In the event that one partner should die, the decision was taken that data collection with the other partner would stop as it was deemed inappropriate to continue unless the other partner indicated otherwise.
Unless otherwise indicated, other reagents were purchased from Sigma-Aldrich, Milan, Italy.
Unless otherwise indicated, other plasmids used in this study are based on the pRS plasmid series [28].
Determinations of estradiol, thyroid hormones, cortisol, DHEA, DHEA-S, melatonin, GH, and insulin-like growth factor-I are not indicated unless other endocrine disorders are suspected based on the clinical signs and symptoms of the patient (12) (Level 2, Grade A).
Purified lipoteichoic acid (LTA) from Staphylococcus aureus and all other reagents (Unless indicated otherwise) were purchased from Sigma.
Human vitronectin, LPS (Escherichia coli O55B5), and all other chemicals unless indicated were from Sigma-Aldrich (St Louis, MO, USA).
Top views of midguts are shown in these panels and in panels of all other figures unless indicated otherwise.
Results are reported as median and interquartile ranges (IQR) for MFI and as the mean ± standard deviation (SD) for other parameters, unless indicated otherwise.
