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In some reactions, 1000-fold excess unlabeled GTP was added to the reaction during hydrolysis ('+excess unlabeled GTP' condition).
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Under unlabeled search conditions, we identified five proteins from the liver and 103 proteins from the brain.
(F) Gel mobility shift analysis of competition of GST-CTCF-ZFs binding to the biotin-labeled M2 by an excess amount of unlabeled M2. Assay condition was the same as in Fig. 1B.
Hearts were perfused with [U-13C]palmitate, [3-13C]lactate, [2-13C]pyruvand and unlabeled glucose; under the conditions of these experiments there is negligible contribution from endogenous triglycerides to unlabeled acetyl-CoA formation [25].
(A ) Time-dependent spectra for [CH3-Ile]p66∆PL with a twofold excess of unlabeled p66 after dimerization conditions were initiated.
This same cross-linked species is observed under turnover conditions with unlabeled substrate, consistent with sulfur insertion at C8 being rate-limiting.
Dissociation rate constants (kd) for all three variants were determined from the time dependence of the decrease in Cy3 fluorescence when the labeled complex is mixed with a large excess of unlabeled EF-Tu, under conditions where dissociation of the labeled complex is rate limiting for formation of unlabeled TC, giving the relative values shown in Table 3.
Each sample was assayed in duplicate, and three samples (from three different infections and extractions) were done for each condition (labeled or unlabeled).
In order to assess the effect of the self-taught learning, we need performance comparison with a system build under the same conditions but without unlabeled data.
ELISA analysis of conditioned media from unlabeled transfected COS cells indicated that Db sMHCI(1 298) was present in the media at ≈ 1.0 nM.
To avoid possible complications that might arise from the very different catastrophe frequencies and shrinking rates, we initially performed these assays under 'catastrophe free' and slow shrinking conditions by using (unlabeled) GTPγS to support assembly.
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