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Dutilh, B. E. et al. A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes.
Dutilh, B.E. et al. A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes.
Primers are often designed to target the desired DNA sequences, however they can target unknown sequences amplifying different organisms.
In this regard, a completely random shRNA library targeting all the genes with known or unknown sequences is of priority.
There is a universe of unknown sequences that are to be discovered and deposited in the database.
DoubleTwist used powerful supercomputers provided by Sun Microsystems to search the raw genome sequence looking for telltale patterns representing the genes and comparing unknown sequences with those of known genes.
This technique was applied to detect and reveal unknown sequences from the large subunit of oxygen-tolerant NiFe-hydrogenase in Hydrogenovibrio marinus.
This method can be widely used in genome walking studies to amplify unknown sequences that are adjacent to known sequences.
In this study, to obtain longer unknown sequences, amplifying long enough single stranded DNA by linear amplification is necessary.
All hits with >97% similarity were downloaded and aligned with the unknown sequences for phylogenetic analysis (Matsunaga et al. 2005).
Black shadow positions on the DNA represent the known sequence, and the residual white parts on the DNA represent unknown sequences.
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