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Peptides containing unknown residues (X) were ignored.
?: unknown residues.
Additionally, a heuristic method is available as a fallback for loading unknown residues or to handle non-standard residue and atom names.
4. PDB chains are removed if the structures have only CA or sequences with length less than 30 or contain too many unknown residues ('X's).
The screening of an epPCR library where the periplasmic domain of PglB Cj had been mutagenized facilitated the identification of previously unknown residues which are relevant for OST activity.
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This scenario could involve another unknown residue functioning as the proton source for the oxirane oxygen.
Direct N-terminal Edman degradation allowed only partial determination of the first six residues: Ile-X-X-Phe-Ile-Gly, where X denotes an unknown residue.
Gaps were excluded from the analysis, and a maximum of one unknown residue was allowed in species other than the bowhead.
CASPER can deal with uncertain linkage positions and unknown residue identities for cases where chemical analysis is incomplete, but the calculations will require more time.
A mechanism was proposed for EI-catalyzed isomerization that involved a histidine conserved between DH and EI domains, as well as an unknown residue that could act as a general base.
In vitro reconstitution of isomerase activity coupled with site-directed mutagenesis revealed that the proposed active site histidine is indeed required for catalysis; however, the unknown residue that acts in cooperation with this histidine could not be determined through mutagenic knockouts of proposed active site residues.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com