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It is worth considering whether the DSS1-protein adducts observed are genuine mixed species or DSS1 oligomers with unknown modifications.
I mean unknown modifications.
7c Mass values of fragment ions from dissociation of unknown RNA with unknown modifications can in principle be aligned as "mass ladders" 17 to reveal the exact mass values of all residues and their sequential arrangement.
This can be due to the incompleteness of the nearest neighbor energy model or explained by the fact that the minimal free energy structure is not necessarily the native one due to unknown modifications or effects of folding kinetics (Higgs and Morgan, 1995).
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Also, there may be an unknown modification near the missing disulfide bond.
The N-terminal alanine residue was found to be blocked by an unknown modification in the mature human protein [10].
So far the exact substrate and product specificities of ASHH2 have not been demonstrated in vitro, possibly because some other unknown modification on the histone tails is a prerequisite for ASHH2's activity.
This isoform was suggested to result from an unknown modification to CDC123 and was linked to its proteasomal degradation [ 47].
The ATA codon frequently appears in these genomes, so either a unique and unknown modification system must exist [ 42], or they import a tRNA.
Unknown modification or editing starting from cytidine at the first anticodon position may be responsible for this. Figure 1 summarizes gene arrangement changes found in acrodontan mitogenomes.
While two separated spots (S1 and S2) identified as one protein could be caused by some unknown modification(s), increased mass and negative charges due to partial phosphorylation of the protein/isoform could lead to the formation of new spots shifting to high mass and low p I areas as indicated on the 2-DE gel (PS1 and PS2).
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