Remarkably, the ribosomal sequences are not uniform, but rather of two types showing only 95% identity in the "universal" region of the 16 s subunit.
Thus, we focused on the sequence N-1155-1238-C, weich we refer to as the MUC1 universal region (Fig. 1a) because it is highly conserved in isoforms of human MUC1.
In the universal region, three predicted B-cell epitopes, No. 3 (CRRKNYG: N-1186-1192-C), No. 4 (YPTYHTH: N-1209-1215-C), aNo.No. 5 (STDRSPY: N-1223-1229-C), highhigh scores in an evaluation based on physicochemical parameters including hydrophilicity, polarity, accessibility and flexibility (Fig. 1b).
Here, we systematically determined the universal folding regions that significantly affect the efficiency of translation in Escherichia coli.
Use of modified nucleotides, e.g. locked nucleic acids (LNA) [29] in the bridge would allow for higher melting temperatures (Tm) for the universal primer regions.
Sequences were viewed using BioEdit [ 69] and edited to remove universal primer regions.
In the present study, we present another convenient approach to find universal exon regions - gene model prediction by Splign using two or more related genomes to define common models.
DNA barcoding is a recent technique that employs one or a few short, universal DNA regions to place sampled individuals into named species and to identify individuals as belonging to putatively undescribed species (http://www.barcodeoflife.org/).org/
Because nuisance and potentially harmful cyanobacteria are cosmopolitan in distribution, and exposure guidelines should therefore be universal, not region specific, we combined the data from all three regions (Queensland, NSW and Florida).
