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Stratagene Universal Mouse Reference RNA was chosen as a control for two-color hybridization [66].
Total RNA from each sample and the universal reference RNA (Universal Mouse Reference RNA, Stratagene, http://www.stratagene.com) were amplified and used in probe preparation as previously described [9].
The fifteen mouse heart tissue cRNA samples were labeled with Cy5 and the Universal Mouse reference cRNA was labeled with Cy3, yielding a total of 5 biological replicates per condition.
Relative transcript levels were determined using a 2 colour reference hybridization design where each RNA sample was labelled with Cyanine 5-CTP (Cy5) and universal mouse reference total RNA (Stratagene, Cedar Creek, TX, USA) was labelled with Cyanine 3-CTP (Cy3) using Agilent Quick Amp Labelling kits (Agilent Tech.
Cy5-CTP-labeled reference target was produced from 2.5 μg of Stratagene Universal Mouse Reference RNA.
Universal mouse Reference RNA (Agilent) was differentially labelled and hybridized to the tissue or cultured samples.
Similar(24)
Other similar studies were done e.g. for T-helper cells [ 20], adipose tissues [ 21], peripheral blood [ 22], various human samples and cell lines [ 23], breast tumor tissues [ 24], breast cancer [ 25], human myocardium [ 26], mouse (universal) [ 27], and human (universal) [ 28].
The notion of a universal remote or mouse as Microsoft Research defined it years ago is now becoming an economic reality, one that de la Vega suggests is recession-immune as of now.
When word leaked on Friday morning that DreamWorks had gotten all hot and heavy with Mickey Mouse, Universal was furious.
The pre-validated hydrolysis probes for TGs-1, 2, 3 and 5 were from human and mouse universal probe library (Roche) and the primers for human and mouse are shown in Table 1 and 2 respectively.
Primers were designed using ProbeFinder version 2.35 for the Mouse Universal ProbeLibrary (http://www.universalprobelibrary.com) from Roche Applied Science and were purchased from MedProbe (primer sequences are listed in the supporting information Table S2).
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