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While universal modifications such as Ψ, mU, tA or mG are found in a large numbers of archaeal, bacterial and eukaryal tRNAs, each kingdom has a set of signature modifications.
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The developed antifouling grafting introduces a universal modification method to generate zwitterionic interfaces on versatile biomaterial substrates, providing great potential for application in medical device coating.
A specific, but universal modification of the WLF-shift procedure due to the strain-induced process of polymer segments changing place is observed and results in a unique frequency-temperature relationship (elastic and rheological simple behaviour).
Ψ-55 is a universal modification inserted in yeast by Pus4p [ 96] and in E. coli by TruB [ 97], both belonging to the same COG0103.
The PHD finger may promote both gene expression and repression through interactions with trimethylated lysine 4 on histone H3 (H3K4), a universal modification seen at the beginning of active genes [ 17, 18].
Also, several studies addressed correlations between intrinsic disorder and the universal Lys modifications, such as acetylation and methylation.
Sodium bisulphite modification of genomic DNA was performed using the CpGenome Universal DNA Modification Kit (Chemicon International, Temecula, CA, USA).
A universal surface modification procedure was developed and performed from an optimized sequence of ultra-violet ozone pretreatment and trimethylamine-catalyzed zwitterionization on a selective case of versatile surfaces including silicon wafer, ceramic glass, titanium, steel, and polystyrene.
To overcome this problem, we have developed a flexible and economic pipeline including PMMA micromachining, surface smoothness improvement, and a universal surface modification for introduction of various functional groups.
One microgram of genomic DNA was treated with bisulfite, which converts the nonmethylated cytosines to thymines, using the CpGenome™ Universal DNA Modification Kit (Chemicon) according to the manufactor's instructions.
The universal base modification tA occurs at position 37 in a subset of tRNAs decoding ANN codons.
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