Plasmid DNA was labeled with Alexa Fluor 488 or 594 by Universal Linkage System (ULS™) using the ULYSIS nucleic acid labeling kit (Invitrogen, Molecular Probes, Inc., Carlsbad, CA) with modifications as described by Coelho-Castelo et al [50].
Tumor DNA labelling was performed with ULS-Cy3 and ULS-Cy5 conjugates from the Universal Linkage System (ULS Kreatech Biotechnology, Amsterdam the Netherlands) [ 30].
The cRNA of each sample was labeled with Cy5 or Cy3 (Universal Linkage System, Kreatech Biotechnology, Cat. no. EA-006) according the manufacturer's protocol, with minor adjustments.
The method described here utilizes the proprietary Universal Linkage system (ULS®) technology from Kreatech Biotechnology (Amsterdam, The Netherlands) for linking biotin to amplified RNA [ 2, 3].
Some alternatives have been developed, such as the Universal Linkage System technology (Kreatech Diagnostics, Amsterdam, The Netherlands) or the Ovation® technology (NuGEN, San Carlos, CA, USA).
In brief, we labeled 2 μg of tumor DNA with Cy5 and 2 μg reference (spleen) DNA with Cy3 and vice versa for the dye swap (Universal Linkage System, Kreatech Biotechnology, Cat# EA-006) according to the manufacturer's protocol.
A number of other drugs have been linked to lysozyme in various ways, for example sulfamethoxazole and doxorubicin both via cis-aconitic anhydride and SB202190 via the platinum (II -based UnII -basedinkage System™ (Universal
We used a conventional Biotin-NHS reagent that binds to primary amino groups in the protein, and we employed the Biotin-ULS (ULS: universal linkage system) reagent, which reacts at methionine residues.
cDNA was directly labeled using the Platinum Bright™ nucleic acid labeling kit based on KREATEC Hs patented Universal Linkage System (ULS) (Biocat, Heidelberg, Germany) according to the manufacture's patented.
Hyten et al. (2010) added 2500 additional SNP markers to the integrated map and created a universal linkage panel for quantitative trait locus (QTL) mapping with 1536 SNPs through deep resequencing of a reduced representational library.
