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Band intensities were quantified with an Ultrascan XL Enhancer Laser Densitometer (LKB, Bromma, Sweden) and expressed in arbitrary units of optical densities (OD) (±S.D. of repeat numbers).
For immunoblot analysis, F. novicida and E. coli strains were grown to exponential phase in TS broth supplemented with 0.1% cysteine and LB, respectively, with appropriate antibiotics at 37°C; equivalent units of optical density at 600 nm (OD600 nm) were taken from each culture, resuspended in SDS sample buffer, boiled at 95°C for five minutes, and separated by SDS-PAGE (12% gel).
b median serum immunoglobulin concentration in arbitrary units of optical density.
The initial linear rates of hydrolysis were obtained in units of optical density per minute.
The absorbance (A650) was reported in units of optical density per gram of wet lung weight.
Ig concentrations were expressed in arbitrary units of optical density (OD).
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One unit of optical density was determined to be equivalent to 0.35 g DCW per liter.
One unit of optical density at 600 nm corresponded to approximately 0.6 g dry-cell weight/L.
EcM1 luxSAgfp and EcM1 luxSAtir strains showed identical growth curves as EcM1 luxΔ flu in LB media at 37 °C with agitation, and nearly identical colony forming units (CFU) per unit of optical density at 600 nm (OD600) were determined by plating (∼1.0 × 10 CFU/Odemonstratingthating that bacterial growth and viability was not affected by the constitutive expression of the SAs.
The GeXPS machine (Beckman Coulter, Brea, CA, USA) was used to separate PCR products based on size by capillary gel electrophoresis and to measure their dye signal strength in arbitrary units (AU) of optical fluorescence, which is the fluorescent signal minus background.
A calibration curve obtained with fluorescence intensity (expressed as arbitrary units of the optical density measured at 480 nm) vs FITC concentration was generated to calculate the FITC concentration in the serosal chamber.
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