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Here we describe an experimental approach to analyze HIV-1 env genes as intact genetic units amplified from plasma virion RNA by single-genome amplification (SGA), followed by direct sequencing of uncloned DNA amplicons.
The mechanisms of airspace injury that mediate adverse patterns of mechanical ventilation include stretching of open lung units, amplified tangential (shearing) forces at the interface between open and closed lung units, and the recurring small airway trauma of tidal breathing.
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Defenseman Michael Del Zotto, who plays the point on the first power-play unit, amplified his coach's comment.
The LNA unit amplifies the input signal with an extremely low level of noise and selects the relevant radio-frequency bandwidth.
The donor transcription units were amplified with primers that introduced terminal BsaI restriction sites, which were subsequently assembled into the universal vector pYW0120 by Golden Gate assembly.
Using primers to amplify the Type B unit, Morgan et al. (31 ) found that the Type A unit was amplified preferentially for human genotype isolates.
The thiolase transcription unit was amplified from C. acetobutylicum genomic DNA using oligonucleotides thlp_for and thlp_rev (Table2).
The signal from each hybridized unit was amplified by attachment of biotinylated label probes at multiple binding sites on the complexes, which in turn bound to streptavidin-conjugated R-phycoerythrin (SAPE) to produce fluorescence.
The ribosomal repeat unit was amplified with Invitrogen's recombinant Taq DNA polymerase, in 5 fragments with an annealing temperature of 50°C and an extension time of one minute.
The signal from each hybridized unit was amplified by attachment of biotinylated label probes at multiple binding sites on the complexes, which were in turn bound to streptavidin-conjugated R-phycoerythrin (SAPE) to produce fluorescence.
The later was made from serial dilutions of RNA prepared by in vitro transcription and the intensity units of scanned amplified bands using software units.
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