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The rvET algorithm couples the phylogenetic tree to the pattern of variation of a pair of residues, viewed as a single evolving unit (Supplementary Fig. S1).
In the structure of the FabG-NADPH complex the electron density for the cofactor is observed in three protein molecules of the asymmetric unit (Supplementary Figure S3A).
Dexamethasone treatment prevented Purkinje cell loss to a significant extent (59% increased in the number of cells per area unit) (Supplementary Fig 1B).
In crystals that belonged to space group C222(1) (Supplementary Table SI), four MglB monomers form a tetramer in the asymmetric unit (Supplementary Figure S3A).
In the first EBNA1/OriP plasmid (called pEB-C5), 5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit (Supplementary information, Figures S1 and S2).
The three following glucose units, however, extend away from the protomer surface and engage in interactions with a neighbouring non-crystallographic symmetry (NCS -related protomer iNCS -relatedtric unit (Suprotomerary Materinl, Fig. S2A).
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The total data set comprised 307 operational taxonomic units (Supplementary Table S1).
These individual models displayed an average RMSEtest value of 0.73 ± 0.05 pGI50 units (Supplementary Table S12).
For the kappa index, the difference between unbiased and original value was only equal to 0.03 units (supplementary Table S3).
However, one wild-type clone of PeSL206/205 was obtained and it showed high lacZ expression levels (∼13,000 Miller units; supplementary fig. S1, Supplementary Material online).
RepeatMasker with the carnivore library was also used to identify microsatellites with a minimum of 15 repeat units (supplementary table S6, Supplementary Material online).
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