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This enhancement appears to be such that the conversion per unit of cell mass is greater rather than being due to an increase in cell mass.
Remarkably, it was demonstrated that β-galactosidase activity per unit of cell biomass was higher in alginate-immobilized than in free-growing cells in the same medium.
Earlier in the growth phase (OD600nm up to about 0.02, or about 2 days) the expression of VHb was correlated with an approximately 30% increase in nitrite conversion per unit of cell mass compared to the untransformed strain; the transformant's advantage decreased gradually to only about 6% by OD600nm of about 0.10 (about 5 days of growth).
We quantified transcription in terms of GFP fluorescence per unit of cell density PFUS1-GFP/OD600 PFUS1-GFP/OD600
In these equations the product by the mass and the division by the cell volume takes into account that the rates r are commonly reported in the literature per unit of dry weight, while the pathway rates f are reported per unit of cell volume.
This data provides a measure of the relative production of secreted protein per unit of cell produced.
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Briefly, each assay was conducted using 1.0 absorbance unit of cells.
After ultrasonication, protein contents of soluble fraction and insoluble fraction corresponding to 0.2 OD600 unit of cells were run on a 12% SDS polyacrylamide gel.
For determination of total cellular poly P, 1 OD680 unit of cells was harvested (2'300 g, 2 min) and the pellet frozen immediately at -20°C for later analysis.
When cultures reached an OD600 one1.2, OD600D600 unit of cells was plated on concanavaline A-coated coverslips and incubated at 30 °C for 1 h.
Analysis in the developing chick hindbrain provided the first evidence that rhombomeres are units of cell lineage.
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