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The plaque size was converted from the original unit in pixel to mm2 by compared it to a standardized round image with known pixel number.
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Change your units from Pixels to Percentage.
For example, southward Y direction as in Feb. and Sep. of 2016 results in the line of sight at around (3E, 10S) where unit is in pixel = 0.012°; this means that the actual line of sight was found at a little different direction from the expected direction by 0.04° eastward and 0.12° southward.
The part of equation ((y-h)/2) (times ) ((tau )/2(^{xi })) is used to convert image pixel to meter unit in the global coordinate (Fig. 9).
This difference in pixel units is converted to metric unit with a constant C which is predefined according to the perspective transformation values before the overall process.
The relative volume of each spot was determined from the spot intensities in pixel units and normalized to the sum of the intensities of all the spots of the gel.
The relative volume of each spot was obtained by determining the spot intensity in pixel units and normalizing that value to the sum of the intensities of all the spots of the gel.
We used an open source video analysis software to measure pupil size in pixel units.
Gels were scanned using an Image Scanner UMAX, Amersham (300 dpi, 12-bit image), which allowed us to obtain spot intensities in pixel units.
This equivalent circular diameter, measured in pixels, was converted to length units by applying the conversion factor between the length measured in pixel units and length measured in micrometers determined from the instrument calibration.
An IMARIS Coloc® (Bitplane, Zurich, Switzerland) module was used to calculate the co-localized voxels (volume unit, analogous to a pixel in two dimension images) between the two channels.
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