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Animals were anesthetized with 1.5 2% isoflurane (in room air), placed into a spinal unit apparatus (Stoelting) and a partial Th12 L1 laminectomy was performed using a dental drill (exposing the dorsal surface of L2-L5 segments).
Schizont-infected RBCs were purified from uninfected and ring-infected RBCs by passage through a size LD column and magnetic unit apparatus (varioMACS; Miltenyi Biotec, Bergisch Gladbach, Germany) [ 49], at 4°C.
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The whole apparatus worked perfectly.
We use the Mini Vertical Unit electrophoresis apparatus from Hoefer.
PowerPac high voltage power supply (Bio-Rad), Mini Vertical Unit electrophoresis apparatus (Hoefer), and Roller mixer (Stuart) were used.
Nitrogen adsorption/desorption isotherms, BET surface areas, total pore volumes and average pore diameter were determined from the corresponding N2 adsorption/desorption isotherms measured at −196 °C using a NOVA 3200 Unit, USA apparatus.
It has two major components, a disposable polymethyl methacrylate (PMMA) micro-reactor and a temperature-regulated optical detection unit (base apparatus) for real-time monitoring of the turbidity changes due to the precipitation of DNA amplification by-product, magnesium pyrophosphate.
Core body temperature was monitored with a rectal probe and maintained at 36°C-37°C 36°C-37°Comeothermic blanket control using(Qingdao Apparatus, Chomeothermic and after the surgery operation.
After the injections, two stainless steel pin electrodes (1-cm gap) connected to an ECM 830 electroporation unit (BTX/Harvard Apparatus, Holliston, MA) were laid on top of the proximal and distal myotendinous junctions.
By 4 p.m., six alarms had been sounded, summoning 225 firefighters and 75 units of apparatus, including water-gushing tower ladders.
Electrophoresis was done with EC-3000 p-series powerandable power and chamber supply units (E.C. Apparatus Corporation) using cellulose acetate strips.
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