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Only the specific tags assigned to genes (A "specific" tag is defined as a tag that uniquely matches to a single gene or that can be resolved to a single gene by taking the lowest position match [ 32]) were used.
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Interestingly, among 19,435 signatures uniquely matched with UniGene clusters, we here identified 1,819 unique signatures matching antisense strands according to the signature classification (see additional file 1 and 4), according to the previous description by Jongeneel et al [ 6].
Of the clean reads, more than 62% matched either to a unique or multiple genomic positions, and 4,106,692 and 3,922,411 uniquely matched reads were used for gene expression analysis of each library.
From a total of 70,619 unique sRNA sequences, they identified 657 differentially expressed (DE) sRNAs that uniquely match tasRNA, which is produced by RDR6, and that are decreased in RDR6 mutants and regarded as provisional true positives.
Do pairs uniquely match the needs of a creative quest?
According to our algorithm definition, all peaks have to be uniquely matched because both compounds had 11 peaks.
For this reason, we also propose to uniquely match spectra peaks, enabling improved differentiation of compound structures through the introduction of long distance peak matching in the metric.
The complicated network can be simplified by using Floyd algorithm, and the initial traveling wave arrival times are uniquely matched with the transmission path.
Further, PRAMEY2/3/6 have more uniquely matched reads (>20), suggesting a higher expression level at these loci.
The fact that 3 STs did not uniquely match geographical lineage should not be overemphasized at this stage being not statistically significant.
Using ELAND (Anthony J. Cox at Illumina) all reads were mapped to the A. fumigatus Af293 genome (GenBank accession numbers NC_007194 to NC_007201 correspond to chromosomes 1 to 8), and all uniquely matching sequences were retained.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com