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In order to collect transcription data from distinct tags matching to a unique transcript or a unique genomic loci without a priori knowledge of genome annotation, we clustered tags previously mapped to Ae. aegypti genome.
The most abundant transcript uniquely expressed in LD has only 558 reads (PGSC0003DMG400026590) whereas the most abundant unique transcript under SD conditions has only 208 reads (PGSC0003DMG400016462).
This study reveals, for the first time, a unique transcript pattern from sorted live amastigotes-hosting DCs that would have been undetectable in unsorted samples.
Our strategy consists of combining and subtracting the input of these various cell types which has produced a unique transcript profile of the squamous carcinoma cell.
culicifacies mosquitoes; we have identified a 383 bp long unique transcript encoding a Drosophila homolog of the quick-to-court protein.
The aim was to generate an EST database containing at least 10,000 unique transcript sequences.
These ESTs were assembled in 12,709 unique transcript sequences and nearly 4,000 sequences were functionally annotated.
The current report describes the construction of an S. gregaria EST database containing 12,709 unique transcript sequences.
Second, the assumption that the long SAGE tags of 21 bp in length are long enough to ensure unique transcript identification is imperfect [41].
In some cases, several supposedly unique transcript sequences matched a single gene, mainly because they matched different regions of the gene.
When ordered by unique transcript (i.e. averaging out probesets that recognize distinct portions of the transcript), p21 was the 10th-most stabilized transcript out of 13,486.
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CEO of Professional Science Editing for Scientists @ prosciediting.com